Effect of small interfering RNA against connective tissue growth factor in TGF-?_1 stimulated human keloid fibroblasts
- VernacularTitle:RNA干扰对人瘢痕疙瘩成纤维细胞TGF-?_1刺激结缔组织生长因子表达的影响
- Author:
Ping ZHAO
;
Shirong LI
- Publication Type:Journal Article
- Keywords:
connective tissue growth factor;
RNA interfering;
human keloid fibroblasts;
transforming growth factor
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of small interfering RNA(siRNA)-expressing plasmid targeting connective tissue growth factor(CTGF) on the expressions of its mRNA and protein in cultured human keloid fibroblasts(hKFs).Methods Constructed siRNA-expressing plasmids targeting CTGF were transfected into hKFs by Dosper package.Then the transfected cells were treated with transforming growth factor 1(TGF-?1,10 ng/ml).Normal hKFs cells and those with TGF-?1 treatment served as normal control and control groups respectively.The expression level of CTGF mRNA and protein were detected by real-time fluorescent quantitative PCR(RQ-PCR) and Western blotting respectively.Results The expression level of CTGF mRNA was only 56.6% of that of the normal control(P
