Benzyl isothiocyanate induces apoptosis of human leukemia cells:molecular mechanism
- VernacularTitle:异硫氰酸苄酯诱导人急性白血病细胞凋亡的分子机制研究
- Author:
Bo CAO
;
Xiaoqin WANG
;
Zegui LI
;
Ning GAO
- Publication Type:Journal Article
- Keywords:
benzyl isothiocyanate;
leukemia;
apoptosis;
myeloid cell leukemia-1
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the apoptotic effect of benzyl isothiocyanate(BITC) on human leukemia cells and investigate its related molecular mechanism.Methods Cells(U937,Jurkat and HL60) were exposed to BITC at various concentrations(0,2,4,6 or 8 ?mol/L) for 6 h or 12 h,or at 8 ?mol/L for different time intervals.The apoptosis was measured using flow cytometry.The apoptotic-related proteins,such as Caspase-3,poly ADP-ribose polymerase(PARP),myeloid cell leukemia-1(Mcl-1) and so on,were determined using Western blot assay.Results BITC induced apoptosis in human leukemia cells in dose-and time-dependent manners.BITC at a dose over 4 ?mol/L began to induce an increased expression of Caspase-3 protein and decreased expression of PARP in U937 cells,and when the dose was 8 ?mol/L,the changes reached their summits respectively.The expression of anti-apoptotic protein Mcl-1 was decreased in U937 cell after exposure of BITC.Conclusion BITC induces apoptosis in human leukemia cells including U937,Jurkat and HL60,and downregulation of Mcl-1 may play an important role in BITC-induced apoptosis.
