Effect of Kidney-jing Deficiency on oxidative stress of male offspring
- VernacularTitle:雄性小鼠肾虚对其雄性仔鼠睾丸组织氧化应激水平的影响
- Author:
Jie SUN
;
Anfang ZHOU
;
Yanyan ZHOU
;
Ting FANG
- Publication Type:Journal Article
- Keywords:
Kidney-jing deficiency;
Animal model;
Offspring;
Oxidative stress;
Kidney-tonifying Recipe
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of kidney-jing deficiency of male mice on the oxidative stress of their male offspring. Methods:Thirty 6-weeks-old Kunming male mice were divided into three groups randomly:the blank control group,the model group and the kidney-tonifying group. The model and the kidney-tonifying groups were stressesd by fear plus excessive sex to establish a kidney-jing deficiency model,and meanwhile the latter were given concentrated solution of Kidney-tonifying recipe intragastrically at the does of 0.16ml/10g. The control and the model groups were treated with physicological saline at the same dose for 21 days. Then all the male mice were mated with the healthy estrous females for 5 days. The malondialdehyde(MDA) ,superoxide dismutase(SOD) and total antioxygen capacity(T-AOC) of testicle of male offsprings were detected at 6 weeks. Results:The level of MDA in model group was higher than that in blank control group and kidney-tonifying group significantly((24.26?6.05) vs(16.54?1.47) ,(15.04?1.50) ?mol/gprot(P
