Studies on hippocampus proteome of rats with syndrome of spleen yin deficiency by two-dimensional gel electrophoresis
- VernacularTitle:脾阴虚证大鼠海马蛋白质组双向凝胶电泳的研究
- Author:
Libin ZHAN
;
Li LIU
;
Xiaoguang LU
;
Xiaoyang GONG
- Publication Type:Journal Article
- Keywords:
Deficiency of spleen yin;
Hippocampus;
Learning and memory abilities;
Proteome;
Two dimensional gel electrophoresis
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To apply two dimensional gel electrophoresis(2-DE) to initially analyze the protein expression in hippocampus of rats with spleen yin deficiency syndrome and to get the theory foundation for investigating the molecular mechanisms of Alzheimer's Disease and spleen yin deficiency syndrome.Methods:Rats model of spleen yin deficiency were set up by combination of improper diet,overstrain and consumption of body fluid.Qualitative changes of the proteome expression in hippocampus were compared between the model group and control group by two-dimensional gel electrophoresis.Results:The model group of spleen yin deficiency had the appearance of spleen yin deficiency,for example,irritability,increment in biting frequency,increase of rectal temperature and drinking water(P
