Regulation of berberine and dioscin in expression of glucose metabolism molecular in trophoblast cells
- VernacularTitle:中药小檗碱、薯芋皂苷对滋养细胞内糖代谢分子表达的调控
- Author:
Yi MA
;
Xiaoke WU
;
Lihui HOU
;
Suping LI
;
Junxiu XING
- Publication Type:Journal Article
- Keywords:
Berberine;
Dioscin;
Trophoblast;
Glucose metabolism;
Protein molecule
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the regulation of berberine and dioscin extracted from traditional Chinese medicine in the expression of protein moleculer related with glucose metabolism in trophoblast cells,such as IRS-1,P1-3K,Glut1,PPAR ?. Methods:To culture the chorion trophoblast cell in the early pregnancy,to induce cells to suffer glucose metabolism obstacle with the use of WortmaninnTake advantage of berberine and dioscin extracted from the Chinese traditional medicine with intervention,simultaneously set the troglitazone(T) and dimthyl(R) for the control group. Detect the gene expression with the use of RT-PCT,simutaniously detect the protein expression in molecular level. Examine the expression in the protein level with the technique of Western blot in combination with the technique of LSM for the expression location of protein moleculer related.Results:① With the induction of WT,the glucose metabolism inside the tropholast cells becomes abnormal,compared with normal(P
