Relationship between changes in endothelial cell specific molecule-1 and renal injury in sepsis rat
- VernacularTitle:脓毒症大鼠ESM-1水平变化及其与肾损害的关系
- Author:
Liang ZHANG
;
Lin CHEN
;
Xiankai HUANG
- Publication Type:Journal Article
- Keywords:
sepsis;
kidney failure,acute;
MODS;
endothelial cell specific molecule
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes in endothelial cell specific molecule-1(ESM-1) in rat with sepsis and the relationship between ESM-1 level and renal injury.Methods Thirty-six SD rats were randomly divided into six groups: normal control group(n=6),sham operation group(n=6);CLP group(n=24),and in the latter group rats were divided into four subgroups according to time after CLP: CLP3h,CLP6h,CLP12h and CLP24h group(6 each).In rats of CLP group cecal ligation and puncture(CLP) was done to produce sepsis,and rats in sham operation group underwent the same surgical procedure as CLP group except the cesum was not ligated and punctured.The changes in ESM-1,blood urea nitrogen(BUN) and creatinine(Cr) level in peripheral blood were determined.Paraffin section and HE staining were used to examine pathological changes in kidney tissue,and immunohistochemisty was used to determine the ESM-1 expression in kidney tissue.Results Compared with normal control group,the serum level of ESM-1 increased significantly in CLP6h,CLP12h and CLP24h group(P
