Regulation of prostaglandin E_2 on orphan nuclear receptor Nurr1 expression in MN9D cells
- VernacularTitle:前列腺素E_2调控MN9D细胞孤儿核受体Nurr1表达的研究
- Author:
Yongmei ZHAO
;
Haiyan ZHANG
;
Qiuyan XU
;
Fengyue LU
- Publication Type:Journal Article
- Keywords:
prostaglandin E2;
Nurr1;
dopaninergic neurons;
tyrosine hydroxylase
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether prostaglandin E2(PGE2)can increase endogenous Nurr1 expression in a dopamine-synthesizing cell line(MN9D) with immature characteristics and to study whether tyrosine hydroxylase(TH) expression is up-regulated in response to Nurr1-overexpression,in order to investigate the role of Nurr1 during dopaninergic neurons development.Methods MN9D cells were treated with 100 ?g?L-1 PGE2 for 2 h to 6 h.The morphology changes of MN9D cells were observed under phase-contrast microscope. The expression of Nurr1 and TH in MN9D cells was analyzed by using immunohistochemical staining and Western blot analysis.Results ① The morphology of MN9D cells did not change significantly following PGE2 treatment for 2 h,4 h and 6h compared with that of MN9D cells left untreated.② Nurr1-positive staining in MN9D cells treated with PGE2 for 2 h,4 h and 6 h was much stronger than that of untreated cells while the percent of TH-positive MN9D cells after PGE2 treatment for 2 h,4 h and 6 h was similar to that of untreated.③ The expression of Nurr1 protein in MN9D cells treatment with PGE2 for 6 h was significantly higher than that of untreated(P
