Staurosporine aglycone at high concentration causes ERK1/2 phosphorylation in rat pulmonary artery smooth muscle cells
- VernacularTitle:高浓度的staurosporine aglycone激活大鼠肺动脉平滑肌细胞中的ERK1/2
- Author:
Jianing ZHANG
;
Xiaojie CHU
;
Changlian LU
;
Chunling WU
;
Hongxia BAO
;
Xiaobo TANG
;
Daling ZHU
- Publication Type:Journal Article
- Keywords:
staurosprine aglycone(SA);
extracellular signal-regulated kinases 1/2(ERK1/2);
PKC;
PKA
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of SA on induction of ERK1/2 activity in rat pulmonary smooth muscle cells(PASMCs).Methods Western blot analysis was employed to identify the activation of ERK1/2 stimulated by SA at different time points and concentrations in cultured rat PASMCs.Results An unexpected observation showed that ERK1/2 phosphorylation was seen after treatment of SA for 2h at a high concentration(30 ?mol?L-1) but not at lower concentration(from 1 nmol?L-1 to 1 ?mol?L-1).Activation of ERK1/2 pathway could be inhibited by an ERK1/2 inhibitor PD98059 or a protein kinase A(PKA) activator isoproterenol.Conclusion Together,these results suggest that SA has a strong dual regulating effect upon ERK1/2 through PKC and/or PKA pathways in rat PASMCs.
