Establishment of Human Multidrug-resistant Bladder Cancer Pumc-91/ADM Cell Line and it’s Biological Characteristics Evaluation
- VernacularTitle:膀胱癌多药耐药细胞株Pumc-91/ADM的建立及其生物学特性评价
- Author:
Min ZHANG
;
Sheng JIN
;
Man ZHANG
- Publication Type:Journal Article
- Keywords:
Bladder;
neoplasms;
Cell line;
Adriamycin (ADM);
Multidrug-resistance
- From:
Journal of Medical Research
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Multidrug-resistance (MDR) is considered to be the major obstacle for cancer chemotherapy.In order to study tumor MDR in vitro, we designed this study to establish human multidrug-resistant Bladder cancer pumc-91/ADM cell line and investigate its biological characteristics. Methods MDR cell line (Pumc-91/ADM) was induced by wise selection on exposure to increasing dose of Adriamycin (ADM).Cell growth was measured and multidrug resistance to multi-anticancer agents was evaluated by MTT Assay.Flow cytometry was performed to determine cell cycle and the ADM concentration of cell line. The expression of MDR-related genes were determined with reverse polymerase chain reaction (RT-PCR). Results Compared to Pumc-91, the Pumc-91/ADM cell had a prolonged doubling time. The number of cells in S-phase was decreased in Pumc-91/ADM while those in G1 and G2 phase increased. The Pumc-91/ADM cell was 10 times more resistant to ADM than the Pumc-91 parent. The Pumc-91/ADM cell exhibited cross-resistance to methotrexate, vincristine, cisplatin, epirubicin. RT-PCR showed that mRNA expression of GST was significantly increased in Pumc-91/ADM. Conclusion Pumc-91/ADM is human multidrug-resistant, and it offers a model with MDR phenotype for the study of MDR in human bladder cancer.
