Extraction optimization study of total alkaloid in Radix Aconiti
- VernacularTitle:川乌中总生物碱提取工艺优化研究
- Author:
Xuexiang LIU
;
Jianwei CHEN
- Publication Type:Journal Article
- Keywords:
Radix Aconiti;
Total alkaloid;
Aconitine;
Hypaconitine;
Mesaconitine;
Extraction process;
HPLC;
Orthogonal design
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction procedure of total alkaloid in Radix Aconiti.Methods:The orthogonal design(L9(34) ) was carried out for the optimum extraction conditions guided by yield of the extract and the content of aconitine,hypaconitine and mesaconitine determined with RP-HPLC method,the mobile phase was MeOH-H2O2-CHCl3-DEA(70∶30∶ 2∶0.1).Results:The best extraction process was as follows:moisten with ammonia test solution,then leach for 24 hours with 15 times of ether.Conclusion:The optimum extraction methods are rational.
