The correlation between zasp gene and idiopathic dilated cardiomyopathy
- VernacularTitle:ZASP基因与特发性扩张型心肌病相关性研究
- Author:
Lin ZHOU
;
Li RAO
;
Bin ZHOU
;
Jigang REN
- Publication Type:Journal Article
- Keywords:
cardiomyopathy,dilated;
ZASP;
gene mutation;
polymorphism
- From:
Chinese Journal of Practical Internal Medicine
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the existance of ZASP(Z-band alternatively spliced PDZ-motif protein)gene mutations in idiopathic dilated cardiomyopathy(IDCM)patients in Chengdu and to study the relationship between this gene and IDCM.Methods Polymerase chain reaction-single-strand conformation polymorphism(SSCP)and DNA sequencing techniques were used to screening the possible mutation site of the ZASP gene exon 4,6,10,in the unrelated Han ethnic population of Chengdu area(including 120 IDCM patients and 100 normal controls).Results The difference of SSCP patterns were found on exon 10 of ZASP gene between IDCM and control groups.The DNA direct sequencing analysis of exon 10 revealed heterozygote G216T and homozygote T216T.G216T was only founded in 28 IDCM patients and 12 controls.T216T was only found 9 patients and 4 controls.Compared with controls,IDCM patients had different frequencies of the GT genotype and T allele(P
