Effects of ?-interferon on proliferation and B7 expression in SPC-A-1 cell line of human lung adenocarcinoma
- VernacularTitle:干扰素-?对人肺腺癌SPC-A-1细胞增殖和B7分子表达的影响
- Author:
Yilin WANG
;
Yong LIN
;
Siqing SUN
- Publication Type:Journal Article
- Keywords:
interferon-?;
SPC-A-1 cell line;
B7-1,B7-2;
apoptosis
- From:
Chinese Journal of Practical Internal Medicine
2006;0(S2):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of IFN-?on expression of B7 molecules in human lung adenocarcinoma cell line cultured in vitro.Methods Human lung adenocarcinoma SPC-A-1 cells were incubated in the medium with IFN-?respectively.Cell proliferation effect was measured by MTT assay;apoptosis was detected with flow cytometry assay;expression of B7-1 and B7-2 mRNA was determined by RT-PCR.Results Compared with those of control group,MTT shows that IFN-?could reduce the SPC-A-1 cell proliferation.FCM shows the apoptosis rate in the IFN-? group was significantly higher,but there is no different changes in each concentration group.Compared with the control group,expression of B7-1 and B7-2 significantly increased in the IFN-?group,and the expression was not correlated with the concentration of IFN-?in each control group.Conclusion IFN-?can inhibit cell proliferation and induce apoptosis in SPC-A-1 cell line.IFN-?markedly enhance the expression of B7-1 and B7-2 in SPC-A-1 line.The apoptosis may be mediated by up-expression of gene B7-1 and B7-2.
