Effect of urotensin Ⅱ on hypertrophy of cardiac myocytes from neonatal SD rats
- VernacularTitle:尾加压素Ⅱ致体外培养乳鼠心肌细胞肥大效应研究
- Author:
Wei LI
;
Aimin LI
;
Ruiwei GUO
;
Pan GAO
- Publication Type:Journal Article
- Keywords:
urotensin Ⅱ;
cardiac myocyte;
hypertrophy;
calcineurin
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism by which urotensin Ⅱ induces hypertrophy of the cultured rat cardiomyocytes. Methods The cultured cardiomyocytes from neonatal SD rats were treated with urotensin Ⅱ, also with cyclosporine A for its blocking effect on urotensin Ⅱ induced cardiomyocytes hypertrophy. The mRNA and protein levels of ?-MHC and CaN were evaluated by real-time PCR and Western blotting, respectively. Results In the cells treated with 10-8 and 10-7 mol/L urotensin Ⅱ, the mRNA and protein levels of ?-MHC and CaN were significantly higher than that of control (P
