Inhibitive and toxic effects of artesunate on murine bone marrow hematopoietic stem and progenitor cells
- VernacularTitle:青蒿琥酯对小鼠骨髓造血干/祖细胞分化的抑制与毒性作用
- Author:
Tianxing LI
;
Lang YAO
- Publication Type:Journal Article
- Keywords:
artesunate;
hematopoietic stem and progenitor cell;
artemisinin;
hematotoxicity
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the influences of artesunate(AS)on murine bone marrow hematopoietic stem and progenitor cells.METHODS:Murine bone marrow hematopoietic stem and progenitor cells were cultured in vitro with different concentration of AS.The numbers of erythrocyte colony-forming unit(CFU-E)or burst-forming unit(BFU-E)in liquid or semisolid culture were counted through light microscope.Apoptosis of cultured cells and the changes of mitochondrial membrane potential were detected by flow cytometry(FCM)analysis,meanwhile,DNA ladder gel electrophoresis were conducted.RESULTS:AS could significantly inhibit the formation of CFU-E and BFU-E(P
