Inhibition of PI3K/PKB signal pathway improves chemotherapeutic effect on gastric carcinoma cell lines
- VernacularTitle:抑制PI3K/PKB信号通路提高胃癌细胞化疗敏感性的研究
- Author:
Xia XIE
;
Qing GAO
;
Yanli WANG
;
Chun TIAN
- Publication Type:Journal Article
- Keywords:
LY294002;
SGC7901 cells;
SGC7901/VCR cells;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe whether the inhibition of PI3K/PKB signal pathway by LY294002[PI3K pathway inhibitor] could improve the sensitivities of human gastric carcinoma cell line SGC7901 and SGC7901/VCR to anti-cancer drugs.Methods The sensitivities of SGC7901 and SGC7901/VCR to chemotherapeutic drug VCR were detected by MTT.The MDR1 and XIAP mRNA expression levels were evaluated by semi-quantitative RT-PCR,and their protein levels were detected by immunocytochemistry.The PKB and phospho-PKB protein levels were detected by Western blot and the apoptosis ratio was detected by flow cytometry.Results 2?10-5mol?L-1LY294002 enhanced the sensitivities of SGC7901 and SGC7901/VCR cells to VCR(P
