Fasudil inhibits neuronal apoptosis after cerebral ischemia/reperfusion injury possibly through the PI3-K/Akt signal pathway
- VernacularTitle:法舒地尔可能通过PI3-K/Akt信号通路抑制大鼠脑缺血/再灌注区神经元的凋亡
- Author:
Jianhua WU
;
Lixin XIE
;
Fang FANG
;
Yunxiang FANG
- Publication Type:Journal Article
- Keywords:
fasudil;
cerebral ischemia;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of fasudil on neuronal apoptosis after cerebral ischemia/reperfusion(I/R) injury in rats.Method Focal cerebral ischemia/reperfusion model in rats was made by transient occlusion of the middle cerebral artery for 90 minutes followed by 24 h reperfusion.The number of neural apoptotic cells was measured by the method of TUNEL staining;Bcl-2,Bax,Caspase-3 protein expressions were observed by immunohistochemical staining;the expression of phospho-MYPT and phospho-Akt protein was determined by Western blot.Result Compared with the MCAO group,fasudil could decrease the number of neural apoptotic cells,upregulate the expression of Bcl-2 and phospho-Akt protein and inhibit the expression of Bax,Caspase-3 and phospho-MYPT protein significantly(P
