The antiproliferation effect of naftopidil in vascular smooth muscle cells of rats
- VernacularTitle:萘哌地尔抗大鼠血管平滑肌细胞增殖的作用
- Author:
Lisheng LI
;
Ansheng SUN
;
Limei YU
;
Qin WU
;
Xienan HUANG
;
Mu YUAN
- Publication Type:Journal Article
- Keywords:
naftopidil;
vascular smooth muscle cells;
noradrenalin;
proliferation
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of naftopidil(Naf) on noradrenalin(NA)-induced proliferation of rats vascular smooth muscle cells (VSMCs),and explore its mechanisms. Methods The cultured VSMCs was induced to proliferate by NA,and effects of Naf on the VSMCs proliferation was tested by MTT assay and cell counting method respectively. The intra-cellular calcium concentration is investigated by fluorimetry,and the expressions of c-myc,c-fos and hypertension related gene-1(HRG-1) mRNA were tested by Real-Time RT-PCR. Results The proliferation of VSMCs induced by NA was inhibited by Naf(10-7~10-6mol?L-1),which diminished clearly VSMCs [Ca2+]i and decreased mRNA expressions of c-myc,c-fos and increased the expression of HRG-1 mRNA. Conclusions Proliferation of VSMCs induced by NA can be inhibited clearly by Naf. The mechanisms may be related to diminishing [Ca2+]i,antagonizing the up-regulation of c-myc and c-fos mRNA expressions by NA and antagonizing the down-regulation of HRG-1 mRNA expression.
