Biological effects of recombinant pcDNA3.0-hugl-1 plasmid on colorectal cancer cell line LS174
- VernacularTitle:重组质粒pcDNA3.0-hugl-1转染结直肠癌细胞后生物学效应的观察
- Author:
Shiyong HUANG
;
Liqiang HAO
;
Ronggui MENG
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
hugl-1 gene;
soft agar colony formation assay;
wound-healing;
adhesion assay;
Transwell invasion
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the biological behaviors of colorectal cancer LS174 cells before and after pcDNA3.0-hugl-1 transfection,so as to investigate the association of hugl-1 with colorectal cancer.Methods:The eukaryotic expression vector pcDNA3.0-hugl-1 was constructed and transfected into LS174 cells.RT-PCR and Western blotting methods were used to analyze the expression of hugl-1 mRNA and protein in LS174 cells before and after transfection.Soft agar colony formation assay,wound-healing experiment,adhesion assay and Matrigel invasion assays were used to study the effects of hugl-1 expression on the proliferation,adhesion,movement and invasion in LS174 cells.Results:The recombinant plasmid pcDNA3.0-hugl-1 was successfully constructed.RT-PCR and Western blotting showed that the hugl-1 expression was higher in cells transfected with pcDNA3.0-hugl-1 than in those un-transfected or empty vector-transfected cells (P
