Influence of disulfiram on the expression of transforming growth factor-?2 in form-deprived eyes in chicks
- VernacularTitle:双硫仑对鸡形觉剥夺性近视眼中转化生长因子-?2表达的影响
- Author:
Fang XIE
;
Yueguo CHEN
- Publication Type:Journal Article
- Keywords:
Disulfiram;
Transforming growth factor beta;
Myopia;
Disease models,animal;
Chickens
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the temporal expression of retinal-choroidal transforming growth factor-?2(TGF-?2) in form-deprived myopia(FDM) chick eyes and study the effect of an inhibitor(disulfiram,DSF) of retinoid acid(RA) synthesis on refractive development and the expression of TGF-?2.Methods:Ninety new hatched white leghorn chicks were divided randomly and equally into 3 groups:unilateral occluded with frosted goggles(Group A);unilateral intravitreal injection of 4 mmol of the non-specific aldehydedehydrogenase inhibitor disulfiram with further treatment with goggles(Group B);unilateral intravitreal injections of 4 mmol of disulfiram without further treatment with goggles(Group C).Refraction and axial length were measured on 1,7 and 14 days after treatment.Expression levels of TGF-?2 and TGF-?2 mRNA in the retinal-choroid layer were analyzed by immunohistochemistry and real-time RT-PCR.Results:In Group A,the form-deprived eyes made by goggles developed myopia(P0.05) with TGF-?2 mRNA level growing on day 7 and decreased on day 14 in group B and decreased on day 1,increased on days 7 and 14 in Group C(P
