Immunoscreening of schistosomulum cDNA library of Schistosoma japonicum and preliminary identification
- VernacularTitle:日本血吸虫童虫cDNA文库的免疫筛选及其生物信息学分析
- Author:
Yunxia ZHANG
;
Xiaonan WANG
;
Weina ZHANG
;
Jijia SHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Schistosomulum;
cDNA library;
Immunoscreening;
Bioinformatics
- From:
Chinese Journal of Schistosomiasis Control
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain genes encoding the novel molecules for diagnosis of schistosomiasis. Methods The cDNA library of Schistosoma japonicum (Sj) schistosomula of day 15 post-infection was screened with positive patients sera,and the inserts of positive clones were sequenced. Then the sequences were compared with all sequences in GenBank database by Internet. The positive clones were analyzed by the software in bioinformatics. Results After three rounds of screening,15 positive clones were obtained,and among them 11 clones had the meaningful sequences,in which 5 genes coding for Sj mitochondrion,1 gene coding for Sj myosin and the others were SjCHGC05315,SjCHGC01371,SjCHGC04782,SjCHGC05166,SjCHGC09769,respectively. Conclusion Immunological screening by using positive patients sera can be used to discover Sj antigen genes with a potential value for diagnosis.
