Experimental Research of Cross Resistance About Paclitaxel Resistant Human Lung Adenocarcinoma Cell
- VernacularTitle:人肺腺癌紫杉醇耐药细胞株交叉耐药性实验研究
- Author:
Jun QIAN
;
Aizhen YANG
;
Huiying CHEN
;
Haijun XU
;
Shukui QIN
- Publication Type:Journal Article
- Keywords:
Lung tumor;
Multidrug resistance;
Paclitaxel;
Cell line
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate paclitaxel cross resistance and provide information for clinical therapy by establishing a paclitaxel resistant human lung adenocarcinoma cell line.Method A paclitaxel resistant human lung adenocarcinoma cell line named with SPC-A1/Taxol was developed by intermittent exposure to gradually increasing concentration of taxol.Resistant index was calculated from median inhibitory concentration(IC50)which was evaluated by MTT assay.Results SPC-A1/Taxol cell line displayed high resistance to taxotere,vinorelbine,vincristine and doxorubicin.It also displayed median or low resistance to camptothecins and podophyllotoxins.No cross resistance was observed to antitumor platinum and antimetabolite drugs and elemene emusion.Conclusion SPC-A1/Taxol cell line is a typical multidrug-resistant cell line in vitro.It could be useful for studing the cross resistance and direct clinical medication.
