Study of Bioassay to Luteinizing Hormone in Serum for Rhesus Monkey
- VernacularTitle:恒河猴促黄体激素生物测定法及应用研究
- Author:
Xunli WANG
- Publication Type:Journal Article
- Keywords:
Rhesus monkey;
Luteinizing hormone;
Bioassay
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The establish a bioassay method to luteinizing hormone in serum for rhesus monkey.Methods With the technique method of bioassay,we measured the content of luteinizing hormone(LH)in serum for the rhesus monkey.Results The sensitivity of the assay was 0.05ng LH/per tube.The intra and inter assay variations were(3.18?1.52)% and(7.56?3.41)%,which proved that the method accuracy was higher.Both the dose-response curve of LH in serum for rhesus monkey and hLH of standard showed the method could be used to examine LH in serum of biology activity for rhesus monkey,which reflected the physiological status of the animals.The recoverable rates of 10,20 and 40ng hLH added to the diluted ram serum were(99.12?4.56)%,(99.31?6.49)% and(101.02?7.58)% respectively,which demonstrated the veracity of the bioassay results.Conclusion The bioactive LH level in pituitary extract is by far higher than that in circulation.
