In Vitro Culture of Nontransformed Cell Lines Derived from Rat Endometrial Epithelium and Stroma.
- Author:
Byung Moon KANG
;
Suk Won LEE
;
Bee Dong CHAE
;
Eun Hee KANG
;
Hyung Sik CHU
;
Chung Hoon KIM
;
Yoon Seok CHANG
;
Joo Hyun NAM
- Publication Type:In Vitro ; Original Article
- MeSH:
Animals;
Blastocyst;
Cell Line*;
Epithelium*;
Fibroblasts;
Gene Expression;
Hypothalamus;
Oxytocin;
Plastics;
Polymerase Chain Reaction;
Rats*;
Stromal Cells;
Uterus
- From:Korean Journal of Fertility and Sterility
1999;26(1):83-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.
