Isolation and identification of tumor-like stem cells from human nasopharyngeal carcinoma cell line
- VernacularTitle:人鼻咽癌细胞株中类肿瘤干细胞的分离、培养及鉴定
- Author:
Xiaofang GUAN
;
Zhong WEN
;
Congxiang SHEN
- Publication Type:Journal Article
- Keywords:
tumor stem cells;
nasopharyngeal neoplasms;
cell culture technigues;
magnetic cell sorting(MACS);
CD133
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CD133 in human nasopharyngeal carcinoma cell line SUNE,and to detect proliferative and differential ability of CD133+ tumor cells in vitro,and to identify tumor-like stem cells in SUNE.Methods Immunocytochemical staining method and flow cytometry were used to detect the expression of putative tumor-initiated cell marker CD133 in nasopharyngeal carcinoma cell line SUNE,and the isolation technique with immunomagnetic beads was applied to purify CD133+ cells.The proliferative ability of CD133+ tumor cells in vitro was estimated by MTT assay,and the proliferative ability of CD133+,CD133-and control SUNE cells were statistically compared.Finally,the immunocytochemical staining method and flow cytometry were used to detect differential ability of CD133+ tumor cells in vitro.Results CD133 was positively expressed only in 0.35% of the cells in nasopharyngeal carcinoma cell line SUNE.In the serum-free medium RPM I1640,the UV optical density of the CD133+ tumor cells enriched with immunomagnetic beads was 0.317?0.007,0.370?0.002 and 0.558?0.004 at 3,5 and 7 days,respectively.Compared with CD133-cells and control SUNE cells,CD133+ cells showed increased proliferative capacity(P
