Effects of ligustrazine on the calcineurin-? expression in lung tissue of rats under simulated weightlessness condition
- VernacularTitle:川芎嗪对模拟失重大鼠肺组织钙调神经磷酸酶-?表达的影响
- Author:
Ye ZHANG
;
Tianzhi LI
;
Changting LIU
- Publication Type:Journal Article
- Keywords:
weightlessness simulation;
lung injury;
calcineurin-?;
TETRAMETHYLPYRAZINE
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the dynamic changes in calcineurin-? expression in lung tissue of rats under simulated weightlessness condition,and the influence of ligustrazine(tetramethylpyrazine) on the changes in its expression.Methods The rat model of simulated weightlessness condition was reproduced by suspending the rat by tail in-30?.Thirty healthy Wistar rats were randomly divided into 3 groups(10 each): control group,suspended group(rat was suspended by tail for 7 days) and ligustrazine group(rat was suspended by tail and ligustrazine was given for 7days).In the ligustrazine group,the ligustrazine was gavaged in a dose of 1.5mg/kg(once a day).The expression of calcineurin-? in lung tissue was determined by immunohisochemistry and Western blotting.Results It was revealed by immunohisochemistry and Western blotting that the level of expression of calcineurin-? in lung tissue of the animals in suspended group was significantly higher that in control group and ligustrazine group(P0.05).Conclusion The level of expression of calcineurin-? in rat lung tissue may be increased obviously under the simulated microgravity condition,while ligustrazine treatment may down-regulate the calcineurin-? expression.
