Release in vitro of dexamethasone sodium phosphate from thermosensitive in situ gels
- VernacularTitle:地塞米松磷酸钠温度敏感原位凝胶的体外释药特性
- Author:
Qing WU
;
Zhaojing ZHU
;
Xinyu LI
;
Lili MA
- Publication Type:Journal Article
- Keywords:
dexamethasone sodium phosphate;
thermosensitive in situ gel;
drug release in vitro;
HPLC
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the release feature of dexamethasone sodium phosphate from thermosensitive in situ gels in vitro. Methods Rotation rheometer was used to measure the changes of viscosity with temperature. The membraneless model was applied in assessing corrosion behavior of gel using a thermostatic shaker (50 r/min) at an amplitude of 2.5 cm, taking phosphate buffered solution (pH 7.2) as releasing media. The release behavior was investigated by HPLC on a C18 reverse column DiamonsilTM (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of triethylamine solution-methanol-acetonitrile (38∶28∶34), pumped at 1.0 ml/min, and the detection wavelength was set at 242 nm. Results When the temperature was near to the sol-gel transition temperature, the viscosity rose suddenly. Taking dexamethasone sodium phosphate (2 ml, pH 7.2) as media, the gel dissolution and drug release rate followed the zero order kinetics, and the cumulative gel dissolution (Q1) and cumulative drug release (Q2) equations were Q1=0.8238t (r=0.999, P
