Research on the intervention of TIMPs-GM6001 in the traumatic proliferative vitreoretinopathy
- VernacularTitle:基质金属蛋白酶抑制剂GM6001干预外伤性增生性玻璃体视网膜病变的研究
- Author:
Guoxing XU
;
Yabing WU
- Publication Type:Journal Article
- Keywords:
traumatic proliferative vitreoretinopathy;
GM6001;
matrix metalloproteinases;
tissue inhibitors of matrix metalloproteinases
- From:
Ophthalmology in China
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of MMP-2, MMP-9 and TIMP-1. TIMP-2 during the course of traumatie pro- liferative vitreoretinopathy (tPVR) in rat retina treated with GM6001 and without GM6001 and evaluate the interventiunal effect uf GM6001 on tPVR. Design Experimental study. Participants 108 SD rats. Methods Rats were divided randomly into three groups: the Ns control group, the tPVR group, the tPVR treated with GM6001 group. The expression of MMP-2. MMP-9 and TIMP-1. TIMP-2 in retina was analyzed by Western Blot on day 1, 3. 7, 14, 21 and 28. Main Outcome Measures The expression of MMP-2, MMP-9, TIMP-2, TIMP-1 in the SD rats' retina of every group. Results The results of Western Blot showed that the expression of MMP-2 in the SD rats' retina of the tPVR group was stronger than the one in other groups at day 3, 7, 14, 21 and 28: the tPVR treated with GM6001 group was weaker than the one in the tPVR group at day 14, 21 and 28d. The expression of MMP-9 in the tPVR group was stronger than the one in other groups at all time; the cireumstance of tPVR treated with GM6001 group was weaker than the one in the tPVR group at day 1, 3, 7, 14 and 21. The rate of MMP-2/TIMP-2 of the SD rats" retina in the tPVR group increased at day 14, 21 and 28, and it was lower in the tPVR treated with GM6001 group compared with the tPVR group. The rate of MMP-9/TIMP-1 in the tPVR group increased at all time, and it degraded in the tPVR treated with GM6001 group as compared with the tPVR group. Conclusions The dishalance of MMP-2 and TIMP-2, MMP-9 and TIMP-1 involves with the course of tPVR. GM6001 playes an important role in in- terfering the course of tPVR by regulating the balance of these cell faetors.
