Inhibition of UVA-induced apoptosis by the polypeptide from Chlamys farreri via regulation of c-jun and COX-2 expression in HaCaT cells
- VernacularTitle:扇贝多肽通过调节c-jun和COX-2抑制UVA诱导的HaCaT细胞凋亡
- Author:
Shenbo GUO
;
Yanxia XING
;
Chunbo WANG
- Publication Type:Journal Article
- Keywords:
ultraviolet A(UVA);
polypeptide from Chlamys farreri(PCF);
HaCaT keratinocytes;
c-jun;
cyclooxygenase-2(COX-2);
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim A UVA-induced apoptotic model of HaCaT cells was established to investigate the impact of UVA on c-jun/cyclooxygenase-2(COX-2)and explore related molecular mechanism of the polypeptide from Chlamys farreri(PCF)protecting HaCaT cells from UVA-induced apoptosis.Methods Cells were divided into five groups:control group,UVA model group,UVA+5.69 mmol?L-1 PCF group,UVA+2.84 mmol?L-1 PCF group,UVA+1.42 mmol?L-1 PCF group.Expression level of c-jun was assayed by Real-Time PCR and Western blot.RT-PCR and Western blot analysis were used to determine the mRNA and protein levels of COX-2.Using agarose gel electrophoresis,the effects of PCF and COX-2 inhibitor celecoxib on UVA-induced apoptosis were also investigated.Results PCF and celecoxib had inhibitory effect on 8 J?cm-2 UVA-induced apoptosis of HaCaT cells.COX-2 mRNA and protein levels increased after UVA radiation and the discrepancy was significant compared with control group(P
