Effects of bistortae ethanol extraction on cell cycle and apoptosis of ECV304 injured by hydrogen peroxide
- VernacularTitle:蚤休醇提物对H_2O_2损伤的ECV304细胞的细胞周期与凋亡的影响
- Author:
Linlin GAO
;
Furong LI
;
Li KANG
;
Yanhong SI
;
Hao WANG
;
Weicheng HU
- Publication Type:Journal Article
- Keywords:
endothelial cell;
ethanol from bistortae;
oxidation;
injury;
cell cycle;
apoptosis;
caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To make ethanol from bistortae(EFB)from traditional Chinese medicine bistortae,and investigate the protection on the injury of the human umbiliar vein endothelial cell(ECV304)induced by hydrogen peroxide(H2O2).Methods ECV304 cells were cultured and divided by five experimental groups including normal,injury model induced by H2O2 and three pretreated groups with EFB;flow cytometry(FCM)methods Annexin V/PI staining and PI staining were used to analyze the effect of the medicine on the apoptosis and cell cycle;reverse transcription PCR(RT-PCR)was used to detect the mRNA expression level of apoptosis associated protein caspase-3 in all experiment-al groups.Results FCM Annexin V/PI staining result showed early and advanced stage apoptosis rate was lower when predisposed than that of the damaged group,FCM PI staining showed there were more cells in G0/G1 stage in oxidation damage group,but less in S stage,and hypodiploid peak could be observed,RT-PCR showed that the expression of caspase-3 was weakened when predisposed by EFB than without this disposal(P
