Effects of BMP-7 on the expression of extracellular matrix in human renal proximal epithelial cells induced by TGF-?_1
- VernacularTitle:BMP-7对TGF-?_1诱导人肾小管上皮细胞外基质表达的影响
- Author:
Yanfang XU
;
Yilan WU
;
Jianxin WAN
;
Caiyun WANG
- Publication Type:Journal Article
- Keywords:
bone morphogenetic proteins;
transforming growth factor-?;
epithelial cells;
extracellular matrix
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of bone morphogenic protein(BMP)-7 on the expression of extracelluar matrix components(ColⅠand FN)in human renal proximal tubular cells(HK-2)induced by transforming growth factor-?1(TGF-?1),and to explore the inhibition of BMP-7 on renal interstitial fibrosis.Methods HK-2 cells were treated with TGF-?1,BMP-7,or a combination of both for 48 h.The expression of FN was assessed by indirect immunofluorescence.RT-PCR and Western blot were used to determine the mRNA and protein expression of FN and ColⅠ?1.Results Indirect immunofluorescence staining showed that FN staining in 3 ?g?L-1 TGF-?1 group was considerably stronger than that in control group.By an addition of 200 ?g?L-1 BMP-7,the expression of FN was significantly inhibited.RT-PCR and Western blot showed the mRNA and protein expression of FN and ColⅠ?1 were up-regulated by TGF-?1 after 48 hours significantly(P
