Elementary inquiry into the mechanism of annexation of bradykinin and papaverine on BTB opening
- VernacularTitle:罂粟碱与缓激肽配伍开放血肿瘤屏障机制的初探
- Author:
Wenfang QIAO
;
Kun LIU
;
Yixue XUE
- Publication Type:Journal Article
- Keywords:
papaverine;
bradykinin;
IL-1?;
blood-tumor barrier;
glioma
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether the annexation of bradykinin and papaverine had some synergetic effects on the opening of blood-tumor barrier,and find out the action of cytokine in this mechanism.Methods 160 female rats were divided into 8 groups:sham operated group;model group,PA group,BK group,PA+BK group,1/2PA+BK group,1/2BK+PA group and 1/2PA+1/2BK group.20 rats were needed in each group.The C6 brain tumor model was built.The drug was pumped into rat's brain via the carotid artery.The opening of the tight junction was detected by electron microscop.And the permeability of the blood-tumor barrier was tested by Evans blue.The expression of IL-1? was tested by Western blot and the expression of IL-1? on/around the capillary in the brain of the cerebral glioma rats was tested by SABC immunochemistry.Results In contrast to sham operated group/model group,the expression of IL-1? in PA group,BK group and PA+BK group increased obviously(P
