Application of Pseudoreplica Technique in Electron Microscope Observation of A?
- VernacularTitle:假复型技术在A?寡聚体电镜检测中的应用研究
- Author:
Ying ZHANG
;
Jingdong SONG
;
Jianguo QU
;
Tao HONG
- Publication Type:Journal Article
- Keywords:
Pseudoreplica technique;
Alzheimer’s disease;
A? oligomer
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of application of pseudoreplica technique in electron microscope observation of A?.Methods With the modification of classical pseudoreplica technique,ADDLs were spotted and concentrated on the gel before negative staining.Results The structures of 5nm to10nm spherical granules and 20 to 30nm coils were visualized by transmission electron microscopy(TEM).Conclusion Pseudoreplica is rapid and effective in ADDLs condensation,negative staining especially in further ultra miero structure observation and research.
