To Preliminary Study the Effect and Mechanism of PUMA Gene to HELA Cell
- VernacularTitle:PUMA基因对HELA的作用及其机制的初步研究
- Author:
Min XIAO
;
Ying JIANG
;
Yongli SHI
- Publication Type:Journal Article
- Keywords:
PUMA;
Apoptosis;
Chondrosome;
AIF;
cyt-c
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminary investigate the cytoactive of HELA cell after PUMA gene promoting HELA cell apoptosis and whether or not the protein of cyt-c,AIF in the chondrosome have participated in the process of PUMA gene inducing HELA cell.Methods we Used AO/EB dyeing to detect the Morphologic change of cell induces by PUMA gene and Western Blot to detect whether or not the position of cyt-c,AIF in the chondrosome have transferred after PUMA gene induces HELA cell apoptosis.Result ①Fluorescence microscope and inverted phase contrast microscope display HELA cell have a series of apoptosis Morphologic change after transfecting PUMA into HELA cell,and this Morphologic change of cell is more obviously after transfecting 48h than after 24h.;②Western Blot display the protein cyt-c and AIF transferred toward to kytoplasm after HELE cell took place apoptosis,the mount of transfecting protein is more obviously after transfecting 48h than after 24h.Conclusion ① PUMA gene have the effect of promoting HELA cell apoptosis;②cyt-c and AIF take part in the process of promoting HELA cell apoptosis,but during this process if the membrane potential of chondrosome was changing need to do some more study to confirm it.
