Elimination of Oxygen Free Radicals in Rat Myocardial Mitochondria by Diazoxide During Hypothermic Preservation
- VernacularTitle:二氮嗪对低温保存诱导的心肌线粒体氧自由基的清除作用
- Author:
Zhikun YAN
;
Mingzhi ZHENG
;
Zhibin HU
- Publication Type:Journal Article
- Keywords:
Diazoxide;
Heart;
Hypothermic preservation;
Superoxide dismutase;
Maleic dialdehyde
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the elimination of oxygen free radicals in rat myocardium mitochondria by diazoxide(DE) during different duration of hypothermic preservation.Methods The Langendorff model of isolated rat heart was used.After stored in 4℃ Celsior solution or Celsior solution containing 30 ?mol/L of DE for different duration(0,3,6,9 or 12h),the activity of the superoxide dismutase(SOD) and the content of maleic dialdehyde(MDA) in heart mitochondrial were measured.The myocardial mitochondria ultrastructure was also evaluated.Results(1) After hypothermic preservation,the activity of SOD was decreased,the content of MDA was increased.The ultrastructure of myocardium mitochondria was also destrored in preserved rat hearts in a time-dependent manner.(2) Compared with the corresponding Celsior solution hypothermic preservation groups,DE significantly improved the above indexes(SOD,MDA and myocardial mitochondria ultrastructure).Conclusion Diazoxide can protect rat myocardial mitochondria from long-term hypothermic preservation injury by direct elimination of oxygen free radials and enhancement of the activity of SOD.
