Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells
- VernacularTitle:不同浓度羊自体血清对骨髓间充质干细胞增殖能力影响的研究
- Author:
Ying ZHANG
;
Liu YANG
;
Xiaojun DUAN
;
Fuyou WANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
autologous serum;
MTT
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of goat autologous serum at different concentrations on the proliferation of bone marrow-derived mesenchymal stem cells(bMSCs) for optimal serum dose.Methods Autologous serum was prepared from 6 goats at 12 months old,and their bMSCs were isolated from bone marrow.Then the obtain bMSCs were cultured in DMEM/F12 medium with supplement of 5%,10%,or 15% of corresponding autologous serum,and 10% fetal bovine serum served as control.The morphology of bMSCs,cells counting and MTT assay were observed or carried out.Results Morphology of the bMSCs in the different groups were similar.The proliferation of 5% autologous serum group was slower compared with that of 10% fetal bovine serum group(P
