Effects of calcitonin gene-related peptide on tibial fracture healing at early stage in rats
- VernacularTitle:降钙素基因相关肽对大鼠胫骨骨折早期愈合的影响
- Author:
Na HAN
;
Dianying ZHANG
;
Tianbing WANG
;
Peixun ZHANG
;
Baoguo JIANG
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide;
bone fracture;
bone morphogenetic protein-2;
rats
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of calcitonin gene-related peptide(CGRP) on early-stage bone fracture healing and on the expression of bone morphogenetic protein-2(BMP-2) in bone tissue of rats.Methods Totally 30 SD rats with tibial fracture were randomly and equally divided into 3 group and received normal saline(control group),CGRP or CGRP receptor antagonist injection intraperitoneally.In 1,2 and 4 weeks later,the fracture sites from the 3 groups were subjected to X-ray scanning,callus measurement and HE staining.The expression of the BMP-2 was detected by immunohistochemical method.Results There was no obvious difference on callus amounts among the 3 groups,but at 2 weeks after treatment,there were significantly more callus in CGRP group with enlargement in the fracture site than in the other 2 groups;The enlargement of CGRP group became minimized with fused cortex and almost normal morphology and size.So were the other 2 groups.Immunohistochemical results showed that exogenous CGRP significantly increased BMP-2 expression,but the expression was decreased significantly after administration of CGRP receptor antagonist.Conclusion Our results suggest that CGRP may participate in the regulation of bone fracture healing,and induce BMP-2 production,implying that BMP-2 may be related functionally with CGRP in the mechanism of bone metabolism.
