Construction of eukaryotic expression plasmid pcDNA3.1(-)/PSMA7 and its expression in A549 cell line
- VernacularTitle:pcDNA3.1(-)/PSMA7真核表达质粒的构建及其在A549细胞中的表达
- Author:
Jiayu TAN
;
Yaling LUO
;
Xiang HUANG
- Publication Type:Journal Article
- Keywords:
PSMA7 protein, human;
plasmids;
eukaryotic cells;
tumor cells, cultured;
A549 cells
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05). The data proved that PSMA7 was overexpressed in pcDNA3.1(-)/PSMA7-transfected A549 cell line, both in mRNA level and in protein level. Conclusion Eukaryotic expression plasmid pcDNA3.1(-)/PSMA7 has been successfully constructed, and the PSMA7 is stably expressed in A549 cell line. This would pave the way for further study of PSMA7.
