Effects of recombinant adenovirus vector containing vp3 gene on murine Hepa1-6 hepatocellular carcinoma model
- VernacularTitle:携带凋亡素基因的重组腺病毒载体对小鼠Hepa1-6肝细胞癌模型的生长抑制作用研究
- Author:
Qing LIU
;
Yongsheng LUO
;
Zhuosheng LAI
- Publication Type:Journal Article
- Keywords:
adenovirus vector;
gene therapy;
carcinomia, hepatocellular
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of recombinant adenovirus vector containing vp3 gene (AdAFPvp3) on c57BL/6 murine hepatocellular carcinoma model. Methods The titers of adenovirus vectors AdAFPvp3 and AdCMV-eGFP were detected. c57BL/6 murine hepatocellular carcinoma models were reproduced by subcutaneous inoculation of murine Hepa1-6 cells, and tumors were monitored for their generation rates. Twenty-four mice were randomly divided into adenovirus vector AdAFPvp3 group, adenovirus vector AdCMV-eGFP control group and PBS control group (8 each) when the tumors grew into 5mm in diameter. AdAFPvp3 (5?108pfu/100?l), AdCMV-eGFP (5?108pfu/100?l) and PBS (100?l) was intratumorally injected, respectively, in the three groups every other day for 2 times. The volume of tumor and the presence of adverse effects were observed. Seven days after treatment, all mice were sacrificed for evaluation of antitumor effect. Tumors, livers and spleens were all harvested for routine pathological examination. At the same time, the way of tumor cell death induced by vp3 in vivo was identified by TUNEL. Results The titers of both adenovirus AdAFPvp3 and adenovirus AdCMV-eGFP were 5?109pfu/ml. The carcinogenesis rate of hepatocellular carcinoma was 100%. Compared with adenovirus AdCMV-eGFP control group and PBS control group, the volume of tumor was diminished evidently in AdAFPvp3 group after intratumoral injection (P
