Modulatory effects of midazolam on the expression of vascular endothelial growth factor after cerebral ischemia-reperfusion injury in Mongolian gerbil
- VernacularTitle:咪唑安定对脑缺血再灌注沙土鼠血管内皮生长因子表达的调节作用
- Author:
Zhenggang GUO
;
Liping LIU
;
Weidong MI
- Publication Type:Journal Article
- Keywords:
brain ischemia;
reperfusion injury;
midazolam;
vascular endothelial growth factors;
positron-emission tomography
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of midazolam on expression of vascular endothelial growth factor (VEGF) of gerbils following total cerebral ischemia-reperfusion injury to look for an experimental basis for the rational clinical use of midazolam. Methods Seventy-two male gerbils (Mongolian gerbil) were randomly assigned into three groups (24 each): sham injury group, injury group and midazolam treatment group. Total cerebral ischemia was reproduced by blocking the bilateral carotid arteries for 10 minutes with bulldog clamps. When reperfusion began, with release of the clamps, 5mg/kg of midazolam was intraperitoneally injected to the animals in midazolam group, and 50ml/kg of normal saline was given by the same way in the gerbils in injury group. Then the parameters listed below were observed: positron emission tomography (PET) images at 6h, 1d, 3d and 7d after reperfusion, and the expression of VEGF in cerebral tissue was immunohistochemically assessed. Results No obvious abnormality was found in the cerebral tissue of sham injury group. For the animals in the injury group and midazolam treatment group, the brain reinfusion area enlarged obviously (P
