Small intestinal submucosa:Preparation and application in cardiomyocyte tissue engineering
- VernacularTitle:小肠黏膜下层的制备及其在心肌组织工程中的应用
- Author:
Yong GUO
;
Xizheng ZHANG
;
Ruixin LI
- Publication Type:Journal Article
- Keywords:
small intestinal submucosa;
stents;
myocytes, cardiac;
tissue engineering
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the small intestinal submucosa (SIS) to be used as a scaffold in constructing engineered cardiac tissue. Methods Small intestinal submucosa of pig was treated with 0.25% trypsin and then 0.5% SDS for 24 hours each to obtain decellularized SIS. The obtained SIS was tested for its mechanical strength by stretching both horizontally and longitudinally with a material test machine. The cytotoxicity and histocompatibility of the material was also examined. Then the cardiomyocytes harvested from 3-day old neonatal rats were seeded on the SIS to construct engineered cardiac tissue sheets. These acquired engineered cardiac tissue sheets were immunohistochemically stained and observed with inversion microscope and transmission electromicroscope to evaluate their characteristics. Results SIS was decellularized completely. The mechanical capability of the decellularized SIS was satisfactory. Under 15% stretching, its strain and stress was nearly linear. SIS showed no cytotoxicity and inflammatory response. After 12 hours, the cardiomyocytes seeded in the SIS scaffold began to beat spontaneously. Two days later, the cardiomyocytes-SIS scaffold composite (engineered cardiac tissue sheet) began to beat spontaneously, and could beat spontaneously for 14 days. The constructed engineered cardiac tissue sheets consisted of layers of cardiomyocytes and with abundant extracellular collagen in the sheet. Conclusions SIS with good mechanical capability and biocompatibility has been prepared successfully, and then the engineered cardiac tissue has been constructed successfully based on the SIS scaffold.
