Effects of Jiaweitaohechengqitang on expression of?-SMA protein in hepatic fibrosis rats induced by CCl_4
- VernacularTitle:加味桃核承气汤对四氯化碳肝纤维化大鼠?-SMA表达的影响
- Author:
Zhiyou ZHAO
;
Gengtin LIN
;
Junjie ZHANG
;
Gengsheng ZHOU
;
Xinchang WANG
- Publication Type:Journal Article
- Keywords:
Hepatic fibrosis;
Jiaweitaohechengqitang;
CCl4;
Rat;
?-SMA
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Jiaweitaohechengtang on expression of?-SMA protein in hepatic fibrosis rats induced by CCl4.Methods:Hepatic fibrosis models were induced by CCl 4 .All the experimental rats were divided into six groups:normal control group,model control group,colchicine positive treatment group,jiaweitaohechenqitang groups with high- dosage,medium-dosage and low-dosage.Drugs were given to rats for 8 weeks by intragastric administration.Scores of hepatic fibrosis were assessed with semiquantitive scoring system(SSS)through tianlanghong staining.Protein expression levels of ?-smooth muscle actin(?-SMA)were determined with immunohistochemical steining method.Results:SSS in model control group were higher than those in normal control group,but lower than those in three treatment groups with jiaweitaohechengqitang. Compared with nornal control group,the protein expression levels of?-SMA in liver tissues in model control group increased markedly,but decreased when it was compared to jiaweitaohechengqitang groups.There was a significant difference between model control group and three treatment groups.Conclusion:Jiaweitaohechengqitang has therapeutic effects on hepatic fibrosis.The mechanism may be ralated to the inhabitition of the activation of HSC.
