Effect of Radix Paeoniae Bubra on NADPH oxidase p22phox,MCP-1 mRNA expression and neointimal hyperplasia after balloon injury in cholesterol-fed rabbits
- VernacularTitle:赤芍药提取物对兔颈动脉介入术后增殖内膜相关基因表达的影响
- Author:
Huimin ZHU
;
Jinxiu FAN
;
Han SHI
;
Ya CHEN
- Publication Type:Journal Article
- Keywords:
Radix paeoniae bubra(RPB);
Balloon injury;
Neointimal formation;
NADPH oxidase p22phox;
MCP-1
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Radix Paeoniae Bubra(RPB)on NADPH oxidase p22phox,monocyte chemotactic protein-1(MCP-1)mRNA expression and neointimal hyperplasia after carotid artery balloon injury in cholesterol-fed rabbits.Methods:The rabbit model of carotid balloon injury was established adopting Clowes method,and treated with extract of RPB.Component of neointima and expression of proliferating cell nuclear antigen(PCNA)and macrophage was determined by immunochemical stain.The collagen of typeⅠwas detected by special staining for blood vessels and the area of neointima was measured by image assay system.Expression of NADPH oxidase p22phox mRNA,MCP-1 mRNA was detected by in situ hybridization and transcription-polymerase chain reaction.Results:RPB can attenuate the neointima area and proliferation of collagen typeⅠinduced by balloon-injury,remarkable prevent the formation of restenosi,and down-regulate expression of NADPH oxidase p22 and MCP-1mRNA significantly and decrease the degree of macrophages infiltration especially in vessel wall of injuring carotid artery.Conclusions:RPB inhibited NADPH oxidase P22Phox and MCP-1 mRNA expression,and modestly reduced neointimal hyperplasia,which might be partly attributed to its antioxidant and inflammatory effects.
