EFFECTS OF SECOISOLARICIRESINOL ON PROLIFERATION OF BREAST CANCER MCF-7 CELLS
- VernacularTitle:开环异落叶松树脂酚对乳腺癌MCF-7细胞增殖的影响
- Author:
Wenbin ZHANG
;
Zhang WANG
;
Ruijin YANG
;
Rongrong LU
;
Shiying XU
- Publication Type:Journal Article
- Keywords:
flax lignan;
secoisolariciresinol;
MCF-7 cells;
proliferation;
apoptosis
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of secoisolariciresinol (SECO) and its metabolites, enterolactone (ENL) and enterodiol (END), on proliferation of MCF-7 cell line and to the probable mechanism. Method Effect of SECO, END, ENL and genistein (GEN) on MCF-7 proliferation was investigated by MTT assay. Cell cycle arrest was measured by flow cytometry (FCM). Cell morphology was observed by optical microscope. The anticarcinogenic mechanism of SECO was analyzed. Results SECO had promotive effect on the cell growth at lower concentration (≤40 ?mol/L) but inhibition effect at higher concentration (100 ?mol/L). ENL and END, however, showed significant inhibition effect at all tested concentrations (10~100?mol/L). The cell cycle progression was arrested at G2/M phase and apoptosis was observed by optical microscope. Conclusion Effect of SECO on the MCF-7 cell proliferation depends on its concentration. Inhibition effect of SECO may relate to its metabolites ENL or END.
