Effect of bFGF on the MCF-7 Cell Cycle with CD44+/CD24-: Promoting the G0/G1-->G2/S Transition.
10.4048/jbc.2012.15.4.388
- Author:
Zhen Lin YANG
1
;
Kai CHENG
;
Zhao Dong HAN
Author Information
1. The First Affilated Hospital of Binzhou Medical University, Binzhou, China. yzhlin@126.com
- Publication Type:Original Article
- Keywords:
Breast neoplasms;
Cell cycle;
Fibroblast growth factors;
Stem cells
- MeSH:
Breast Neoplasms;
Cell Cycle;
Cell Proliferation;
Cell Transformation, Neoplastic;
Fibroblast Growth Factor 2;
Fibroblast Growth Factors;
Fluorescence;
MCF-7 Cells;
Mitosis;
Phase Transition;
Stem Cells
- From:Journal of Breast Cancer
2012;15(4):388-392
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44+/CD24-. METHODS: Cancer-initiating cells were propagated as mammospheres. The CD44+/CD24- subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations. RESULTS: Differences in the CD44+/CD24- cell proliferation under different bFGF concentrations were observed (p=0.001). When the bFGF concentration was increased, the proportion of CD44+/CD24- at G0/G1 decreased (p=0.023). CONCLUSION: We conclude that bFGF may sustain CD44+/CD24- cell proliferation and could promote cell progression through the G0/G1-->G2/S phase transition.
