Effects of rosiglitazone on the production and degradation of extracellular matrix of glomerular mesangial cells incubated with high concentration of glucose
- VernacularTitle:罗格列酮对高糖培养肾小球系膜细胞胞外基质产生及降解的影响
- Author:
Liansong NI
;
Jiena JIN
;
Jingchen ZHENG
;
Feixia SHEN
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
rosiglitazone;
mesangial cells
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the protection mechanisms of rosiglitazone on diabetic nephropathy.METHODS:Rat mesangial cells(MC) were incubated in media containing 5.5 mmol/L normal control glucose,25 mmol/L high concentration glucose,25 mmol/L glucose +20 ?mol/L rosiglitazone maleate.Cells proliferation were assessed by CCK-8.Synthesis of fibronectin(FN),type Ⅳ collagen(Col-Ⅳ),transforming growth factor-?1(TGF-?1) and matrix metalloproteinase inhibitor-1(TIMP-1) in supernatant were determined by ELISA method,the activities of matrix metalloproteinase-2,9(MMP-2,9) in supernatant were determined by gelatinase zymography.RESULTS:Compared with control group,MC cultured with high concentration glucose showed a high growth rate and increased synthesis of Col-Ⅳand FN,decreased the activities of MMP-2 and MMP-9,and increased secretion of TGF-?1 and TIMP-1.Compared with high glucose group,these changes could be reversed by rosiglitazone intervention.CONCLUSION:Rosiglitazone could inhibit high concentration glucose-induced proliferation of mesangial cells,decrease synthesis of extracellular matrix,and increase degradation of extracellular matrix.
