Association between genetic polymorphism of CYP3A5 and blood concentration/dose ratio of cyclosporine combined with calcium channel blockers in renal transplant patients
- VernacularTitle:钙通道阻滞剂对肾移植患者环孢素血药浓度/剂量的影响与CYP3A5基因多态性有关
- Author:
Daohua SHI
;
Zhijie LIN
- Publication Type:Journal Article
- Keywords:
kidney transplantation;
cyclosporine;
calcium channel blockers;
CYP3A5;
genetic polymorphisms
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the relationship between the genetic polymorphism of CYP3A5 gene and the blood concentration/dose(C/D) ratio of cyclosporine combined with calcium channel blockers(CCBs) in renal transplant patients.METHODS:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis was applied to determine the genotype of CYP3A5 in 69 renal transplant patients.The blood concentration/dose(C/D) ratio of cyclosporine was compared in patients coadministrated of CCBs with different genetic polymorphism of CYP3A5.RESULTS:The cases of genotype of CYP3A51/1,CYP3A51/3 and CYP3A53/3 were 8,23 and 38,respectively.The mutation rate of CYP3A53 in renal transplant patients was 71.74%.The CsA C/D ratio of patients of CYP3A51/1 and CYP3A51/3 were 1.73 and 2.43 times higher than that in patients of CYP3A53/3.The CsA C/D ratio of patients(CYP3A51/1,CYP3A51/3) combined with CCBs had been obviously elevated(19.90?7.66 vs 36.01?8.18,34.33?7.87 vs 46.16?9.63 ng?mL-1 per mg?kg-1;P
