Effects of Zhiling capsule on apoptosis and expressions of hTERT,CD44 in small cell lung cancer cell line NCI-H446
- VernacularTitle:志苓胶囊对小细胞肺癌细胞株NCI-H446细胞凋亡及h-TERT、CD44表达的影响
- Author:
Yingyu CHEN
;
Yunling PAN
;
Zhihong ZHENG
;
Mingji PAN
;
Jianda HU
;
Tingbo LIU
- Publication Type:Journal Article
- Keywords:
small cell lung cancer cell line NCI-H446;
Zhiling capsule;
apoptosis;
hTERT;
CD44
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanisms of the apoptosis induction and the effects of adhesion suppression of Zhiling capsule (ZLJN) in small cell lung cancer cell line NCI-H446.Methods According to the different components of ZLJN,NCI-H446 cells were treated with traditional Chinese medicine,western medicine and ZLJN composite groups.Apoptotic cells were tested by light microscopy,Hochest33258 staining method.The mRNA and protein expressions of bcl-2,bax and hTERT were analyzed by RT-PCR and Western blot respectively.The expressions of CD44 were detected by flow cytometry.Results After NCI-H446 cells were treated with different drug groups,The morphological changes of apoptotic cells were found by light microscopy and Hochest33258 staining method.The mRNA and protein expressions of bcl-2 were down-regulated while the expressions of bax were up-regulated compared to the control groups(P
