Paeoniflorin increases gene expression of erythropoietin through adenosine A1 and A2a receptors in hypoxia
- VernacularTitle:芍药苷通过激活腺苷A1和A2a受体促进低氧条件下EPO基因的表达
- Author:
Kaiqi ZONG
;
Zengchun MA
;
Yue GAO
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
adenosine receptor A1;
adenosine receptor A2a;
erythropoietin;
PI-3K;
hypoxia
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study molecular mechanisms of the effects of paeoniflorin on increasing expression of erythropoietin.Method RT-PCR and Western blot were used the investigate the influences of paeoniflorin,PI-3 kinase inhibitor (LY294002 30 ?mol?L-1),antagonist of adenosine A1 receptors(DPCPX 10 ?mol?L-1),agonist of A1 receptors(CPA 1 ?mol?L-1) and antagonist of adenosine A2a receptors(SCH58261 0.2 ?mol?L-1) on expression of erythropoietin in hypoxia and normoxia.Results Paeoniflorin decreases the expressions of erythropoietin in normoxia.However, paeoniflorin(2 ?mol?L-1) significantly increases the expression of erythropoietin mRNA after exposure to hypoxia environment from 8 to 24 hours in HepG2 cells.And this phenomenon is inhibited by LY294002,DPCPX and SCH58261.Conclusion Paeoniflorin increases erythropoietin mRNA levels by activating PI-3 kinase pathway through adenosine A1 and A2a receptors.
