Protective effect and its molecular mechanism of gross saponin tribulus terrestris on apoptosis of PC12 cells
- VernacularTitle:蒺藜皂苷对PC12细胞凋亡的保护作用及机制
- Author:
Enping JIANG
;
Hong LI
;
Yingshi JI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
GSTT;
PC12 cells;
H2O2;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of gross saponin tribulus terrestris(GSTT) on the apoptosis of PC12 cells was induced by H2O2 and its mechanism.Methods Apoptosis of PC12 cells was induced by H2O2 at the concentration of 300 ?mol?L-1.The cell activity was determined by MTT.Cells were observed by inverted phase contrast microscope.Hochest33258 staining was detected by fluorescence microscope.The subdiploid peaks showing cell apoptosis rate was detected by flow cytometry.Protein of Bcl-2 and Bax was detected by Western blot.Results Compared with model group,the survival rate of PC12 cells increased (P
