The effect of hydrogen sulfide donor on the pathogenesis of atherosclerosis in apolipoprotein E knock out mice
- VernacularTitle:硫化氢供体对Apo E基因敲除小鼠动脉粥样硬化的调节作用
- Author:
Yanfei WANG
;
Chaoshu TANG
;
Junbao DU
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
atherosclerosis;
apolipoprotein E knock out mice
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of hydrogen sulfide (H2S) donor on the pathogenesis of atherosclerosis in apolipoprotein E knock out mice (Apo E-/-).Methods 6-week old C57BL/6J and Apo E-/-mice were divided into normal group, Apo E-/-group and Apo E-/-+ sodium hydrosulfide(NaHS)group,with 8 mice in each group. They were fed chow diet until 16-week old. The serum levels of total cholesterol, low density lipoprotein and high density lipoprotein were determined and the changes of atherosclerosis plaque size were observed. Sulfide electrode-based method was used to measure the serum level of H2S.Results The serum levels of total cholesterol[(12.59?3.11 vs 2.32?0.40) ?mol?L-1]and low density lipoprotein[(1.33?0.43 vs 0.13?0.03) ?mol?L-1] in Apo E-/-mice were significantly higher than those in normal group, while the serum levels of high density lipoprotein[(0.45?0.13 vs 1.49?0.21) ?mol?L-1]and H2S in Apo E-/-mice[(44.64?4.52 vs 57.69?7.03) ?mol?L-1]were significantly lower than those in normal group. Meanwhile, the atherosclerosis plaque[(139316.6?30362.93 vs 0) ?m2] was obvious in Apo E-/-mice. After NaHS was administrated, there were no obvious changes in the serum levels of total cholesterol, low density lipoprotein and high density lipoprotein in Apo E-/-mice, while the serum level of H2S[(52.21?7.24 vs 44.64?4.52) ?mol?L-1]increased significantly and the size of atherosclerosis plaque[(85927.84?1922.73 vs 139316.6?30362.93) ?m2]diminished significantly.Conclusions H2S postponed the proceeding of atherosclerosis and diminished the size of atherosclerosis plaque.
